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目的比较3种人芽囊原虫病原学检查方法的检出效果。方法收集腹泻组400例、健康对照组394例送检粪便标本,分别采用生理盐水涂片法、碘液染色法和改良酸醚离心沉淀法检查人芽囊原虫,并观察镜下形态。结果腹泻患者粪便标本3种方法阳性检出率粪便为11.50%、12.75%和20.75%;改良酸醚离心沉淀法检出率高于其他两种方法(χ2=15.804,P<0.05),且该法检出密度亦明显高于其他两种方法(χ2=144.715,P<0.05)。健康对照组改良酸醚离心沉淀法阳性检出率(1.52%)和检出密度最高。粪便中人芽囊原虫最常见为空泡型,其次为颗粒型。结论临床检测人芽囊原虫时,应根据实际需要和检测条件选取合适的检查方法。
Objective To compare the detection results of three methods of pathogen detection of Blastocystis. Methods 400 cases of diarrhea group and 394 healthy control group were collected for stool samples. The stool specimens were examined by saline smear method, iodine solution staining and modified acid-ether centrifugation method, respectively. Results The positive rates of stool specimens in diarrhea patients were 11.50%, 12.75% and 20.75%, respectively. The detection rate of modified acid-ether centrifugation was higher than that of the other two methods (χ2 = 15.804, P <0.05) The density of the method was also significantly higher than the other two methods (χ2 = 144.715, P <0.05). In the healthy control group, the positive detection rate (1.52%) of the improved acid ether centrifugal precipitation method and the highest detection density were obtained. Human bud bursa in the most common vacuolar vacuoles, followed by granular. Conclusion In clinical examination of Blastocystis protozoan, we should select the appropriate test method according to the actual needs and testing conditions.