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目的探讨内皮素1对HEP 2细胞有丝分裂的影响。方法采用RT-PCR及免疫组化Dako EnVision法检测HEP 2细胞内皮素受体表达情况;3H-胸腺嘧啶核苷渗入法检测不同浓度和作用时间内皮素1对HEP 2细胞有丝分裂的影响。结果HEP 2细胞存在内皮素受体基因及蛋白表达;内皮素1具有明显促HEP2细胞增殖作用,且其促增殖作用呈浓度依赖性。结论内皮素1能有效促进HEP 2细胞有丝分裂,对喉癌的演进可能有重要调控作用。
Objective To investigate the effect of endothelin-1 on mitosis in HEP 2 cells. Methods The expression of endothelin receptor in HEP 2 cells was detected by RT-PCR and Dako EnVision immunohistochemistry. The effect of endothelin-1 on mitosis of HEP 2 cells was detected by 3H-thymidine incorporation. Results Endothelin receptor gene and protein expression existed in HEP 2 cells. Endothelin 1 had a significant effect on promoting proliferation of HEP2 cells in a concentration-dependent manner. Conclusion Endothelin 1 can effectively promote mitosis of HEP 2 cells and may play an important regulatory role in the development of laryngeal carcinoma.