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采取针刺法造成实验大白鼠脑干损伤，用免疫组织化学ＬＳＡＢ法检测大脑、中脑、桥脑及延髓等不同部位脑组织中胶质原纤维酸性蛋白（ＧＦＡＰ）的改变。结果发现，生前损伤３０ｍｉｎ，大脑顶部灰质及中脑腹例中央部的ＧＦＡＰ阳性星形胶质细胞数目增多；生前损伤６０ｍｉｎ，除大脑顶部灰质及中脑腹侧中央部外．大脑脑室角周边部、桥脑背侧中央部、延髓腹侧及背侧中央部的ＧＦＡＰ阳性星形胶质细胞数目亦增多。而死后损伤者，ＧＦＡＰ阳性星形胶质细胞数目不增多。说明ＧＦＡＰ阳性星形胶质细胞数目的改变可区别脑组织的生前损伤和死后改变，并可作为脑干损伤早期的诊断指标。
The acupuncture was used to induce the injury of brain stem in experimental rats. The changes of glial fibrillary acidic protein (GFAP) in different brain regions such as brain, midbrain, pons and medulla oblongata were detected by immunohistochemistry LSAB. The results showed that the number of GFAP positive astrocytes in the central part of cerebrum at the top of gray matter and midbrain was increased after prenatal injury was 30min. The anterior deformity was 60min, except for the gray matter in the top of cerebrum and ventral midbrain. The number of GFAP-positive astrocytes in the periventricular portion of the brain, the dorsal midpoint of the pons, the medulla oblongata and the dorsal middling increased. After injury, the number of GFAP positive astrocytes did not increase. The changes of GFAP positive astrocytes can distinguish preimplantation and post-mortem changes of brain tissue, and can be used as a diagnostic indicator of early brain injury.