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设计并开发出用于DNA聚合酶链式反应(PCR)的微流体芯片温度控制系统。采用具有光学透明的PDMS和ITO导电玻璃,设计制作了温度可控全透明PDMS微流体芯片。系统由硬件电路搭建和控制软件编程共同实现。温度信号由AD590采集,经过CD4051BE选通,传送给ATmega16L,单片机通过比较温度采集值与设置值,得到控制指令,并控制驱动电路。实验结果表明,该系统完全满足设计要求,温度控制精度高,系统体积小,便于PCR扩增实验。
Designed and developed a microfluidic chip temperature control system for DNA polymerase chain reaction (PCR). Transparent PDMS and ITO conductive glass were used to design a temperature-controllable and fully transparent PDMS microfluidic chip. The system is realized by hardware circuit and control software programming. The temperature signal is collected by AD590, after CD4051BE gating, convey to ATmega16L, the one-chip computer compares the temperature to gather value and set value, get the control order, and control the drive circuit. The experimental results show that the system fully meets the design requirements, high temperature control precision, small system size, easy to PCR amplification experiments.