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单向不亲和对于研究种间花粉不亲和及自交不亲和机制有重要意义。以潘那利番茄‘LA0716’渐渗系群体中的单向不亲和株系为主要材料,通过花粉萌发试验,正、反交试验和花粉管在雌蕊中的伸长研究,发现单向不亲和株系的花粉活力正常,其花粉与普通栽培番茄杂交可正常结实,但是其雌蕊不能接受自身或栽培番茄的花粉。然而,将秘鲁番茄‘LA3858’的花粉授予单向不亲和株系,花粉能够正常萌发,花粉管正常伸长和受精,但是杂交种子败育。利用20个分子标记对BC5S3群体的1 000株植株进行遗传分析,结合表型鉴定结果,将单向不亲和基因UI3a精细定位于第3号染色体上分子标记C2_At3g10220和1k-1294之间,物理距离为349 kb,该区域编码37个基因。
One-way incompatibility is of great importance for the study of the mechanism of inter-species pollen incompatibility and self-incompatibility. Taking unilateral incompatible lines of tomato ’LA0716’ introgressed lines as the main material, we studied the pollen germination test, the positive and negative crosses and the pollen tube elongation in the pistil and found that the unidirectional The progeny lines have normal pollen viability and their pollen can be crossed normally with common cultivated tomatoes but their pistils can not accept their own or cultivated tomato pollen. However, pollen from the Peruvian tomato ’LA3858’ was awarded to unidirectional incompatible lines, normal germination of pollen, normal elongation and fertilization of pollen tubes, but aborted hybrid seeds. Genetic analysis of 1000 plants in the BC5S3 population was carried out using 20 molecular markers. Based on the phenotypic characterization results, the unidirectional UI3a was finely mapped between the molecular markers C2_At3g10220 and 1k-1294 on chromosome 3, and the physical The distance is 349 kb and the region encodes 37 genes.