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目的观察小檗碱对T2DM地鼠脂肪组织肝X受体(LXR)及其靶基因表达的影响。方法以高脂饮食结合小剂量STZ的方法建立IR及T2DM地鼠模型。成模后随机分成正常对照(Con)组、IR组、T2DM组、小檗碱治疗(BBR)组,治疗9周。结果与T2DM组相比,BBR组FPG[(13.10±0.93)vs(6.19±0.84)mmol/L]、OGTT 2hPG[(15.49±1.31)vs(8.83±1.28)mmol/L]、HOMA-IR[(14.80±2.10)vs(7.40±1.40)]降低(P<0.05);RT-PCR结果提示,LXRα[(-5.56±-1.23)vs(3.29±0.68)]、LXRβ[(3.09±0.45)vs(-5.49±-0.95)]基因表达上调,并伴随着LXR靶基因表达的改变(P<0.05);Western blot结果提示,LXRα[(0.11±0.03)vs(0.68±0.12)]、LXRβ[(0.13±0.03)vs(0.70±0.14)]蛋白表达上调(P<0.05)。结论 LXR及其靶基因参与小檗碱治疗T2DM地鼠脂诱性脂肪组织IR的分子机制。
Objective To observe the effects of berberine on the expression of liver X receptor (LXR) and its target genes in adipose tissue of T2DM hamsters. Methods High-fat diet combined with low-dose STZ method to establish IR and T2DM hamster model. After modeling, they were randomly divided into normal control (Con) group, IR group, T2DM group and BBR group for 9 weeks. Results Compared with T2DM group, FPG [(13.10 ± 0.93) vs (6.19 ± 0.84) mmol / L], OGTT 2hPG [(15.49 ± 1.31) vs (8.83 ± 1.28) mmol / L] (P <0.05). The results of RT-PCR indicated that LXRα [(- 5.56 ± 1.23) vs (3.29 ± 0.68)], LXRβ [(3.09 ± 0.45) vs (-5.49 ± -0.95)], and the expression of LXR target gene was up-regulated (P <0.05). The results of Western blot suggested that LXRα [(0.11 ± 0.03) vs (0.68 ± 0.12) 0.13 ± 0.03) vs (0.70 ± 0.14)] (P <0.05). Conclusions LXR and its target genes are involved in the molecular mechanism of berberine in the induction of IR in murine adipose tissue of T2DM rats.