Experimental varicocele was created in male rats by partial lig-ation of left renal vein (VC) and control rats received a sham oper-ation (SO). Changes in AQP7mRNA expression and testicularfunction were observed. The results are as following: 1.AQP7mRNA expression in left testis of VC rats decreased signifi-cantly, while increased in the right, when compared with SO. 2.Weights of left testis in VC rats were significantly lower than thosein SO (P<0.05) and lower than those of the right (P>0.05);no difference was found between right testes in two groups. In VC,especially in left testes, there were sloughing spermatogenous cellsin slight and moderate atrophied seminiferous tubules, and edema inintestitial tissue as well. It suggests that left varicocele results indysfunction of spermatogenesis in rat testes, especially in the lefttestis. Obvious difference in AQP7mRNA expression suggestedchanges of water metabolism might be one of reasons for distur-bance of spermatogenesis, while active metabolism in righ Experimental varicocele was created in male rats by partial lig-ation of left renal vein (VC) and control rats received a sham oper-ation (SO). Changes in AQP7 mRNA expression and testicular function were observed. The results are as follows: 1. AQP7 mRNA expression in left testis of VC rats decreased signifi-cantly, while increased in the right, when compared with SO. 2. Weights of left testis in VC rats were significantly lower than those of SO (P <0.05) and lower than those of the right No difference was found between right testes in two groups. In VC, especially in left testes, there were sloughing spermatogenous cells slight slight and moderate atrophied seminiferous tubules, and edema inintestitial tissue as well. It suggests that left varicocele results indysfunctionofspermatogenesis in rat testes, especially in the left testis. Obvious difference in AQP7 mRNA expression suggested changes of water metabolism might be one of reasons for distur-bance of spermatogenesis, while active metabolism in righ