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目的:探讨重组25kDa猪釉原蛋白(recombinant porcine amelogenin,rPAm)对人牙龈上皮细胞(human gingival epithelial cells,HGEC)黏附、增殖和迁移的影响。方法:采用不同浓度的rPAm(0、5、10、20μg/mL)刺激第2代HGEC,在相应的时间点,应用细胞计数法测定黏附和增殖的效果,以创面愈合模型测定细胞迁移效果,采用GraphPad Prism软件对数据进行统计学分析。结果:在黏附实验中,rPAm对HGEC的黏附有抑制作用,且效果随着时间的递增和rPAm浓度的增高而加强;在增殖实验中,随着时间的递增和rPAm浓度的增高,HGEC增殖能力下降;在迁移实验中,rPAm能抑制HGEC迁移,20μg/mL效果最好,24h后呈现剂量和时间依赖性。结论:rPAm能显著抑制人牙龈上皮细胞的黏附、增殖和迁移,存在剂量和时间依赖关系。
Objective: To investigate the effect of recombinant porcine amelogenin (rPAm) on the adhesion, proliferation and migration of human gingival epithelial cells (HGEC). Methods: The second generation HGEC was stimulated with different concentrations of rPAm (0, 5, 10, 20μg / mL). At the corresponding time points, the cell adhesion assay was used to determine the effect of adhesion and proliferation. The wound healing model was used to determine the effect of cell migration. Data were statistically analyzed using GraphPad Prism software. Results: In the adhesion experiment, rPAm inhibited the adhesion of HGEC and the effect was enhanced with the increasing of time and rPAm concentration. In proliferation experiment, with the increasing of time and rPAm concentration, the proliferation ability of HGEC In the migration experiment, rPAm can inhibit the migration of HGEC, 20μg / mL the best, 24h after a dose and time-dependent manner. Conclusion: rPAm can significantly inhibit the adhesion, proliferation and migration of human gingival epithelial cells in a time-and dose-dependent manner.