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目的观察甜菜碱对癫痫大鼠血清中同型半胱氨酸以及对海马γ-氨基丁酸及其受体的影响。方法将健康雄性Wistar大鼠随机分为正常对照组(腹腔注射生理盐水,1.0 mL生理盐水灌胃),癫痫组(腹腔注射戊四氮,1.0 mL生理盐水灌胃),甜菜碱高、中、低浓度组(腹腔注射戊四氮,甜菜碱灌胃),丙戊酸钠组(腹腔注射戊四氮,丙戊酸钠灌胃),实验结束后大鼠眼眶取血检测血清中同型半胱氨酸的含量;在低温条件下迅速取脑,ELISA实验检测γ-氨基丁酸含量的变化;兔疫荧光和Westernbloting检测海马区γ-氨基丁酸受体的变化;取肝组织进行HE染色观察组织形态变化。结果癫痫组同型半胱氨酸的含量与正常组比较显著降低(P<0.01),甜菜碱高、低浓度组同型半胱氨酸含量与癫痫组比较明显降低(P<0.05);ELISA结果表明癫痫组γ-氨基丁酸的含量与正常对照组相比显著下降(P<0.01)。甜菜碱高、中浓度组γ-氨基丁酸的含量与癫痫组比较显著增高(P<0.01);免疫荧光与Western bloting检测结果,癫痫组γ-氨基丁酸受体的表达与正常对照组相比显著降低(P<0.01),甜菜碱高、中、低浓度组较癫痫组显著升高(P<0.01);HE染色结果,丙戊酸钠组动物肝脏组织镜下观察可见明显的空泡变性(P<0.05)。结论甜菜碱具有较好的抗癫痫作用,且无肝毒性。
Objective To observe the effects of betaine on serum homocysteine and γ-aminobutyric acid and its receptor in hippocampus of epileptic rats. Methods Healthy male Wistar rats were randomly divided into normal control group (intraperitoneal injection of saline, 1.0 mL saline), epilepsy group (intraperitoneal injection of pentylenetetrazol, 1.0 mL saline), high, Low concentration group (intraperitoneal injection of pentylenetetrazol, betaine gavage), sodium valproate group (intraperitoneal injection of pentylenetetrazol, sodium valproate intragastric administration), after the end of the experiment rat orbital blood to detect serum homocysteine The content of γ-aminobutyric acid in the hippocampus was detected by immunofluorescence staining and Western blotting. The liver tissues were harvested for HE staining Tissue morphological changes. Results The content of homocysteine in epilepsy group was significantly lower than that in normal group (P <0.01). The content of homocysteine in high and low concentration of betaine was significantly lower than that in epilepsy group (P <0.05) The content of γ-aminobutyric acid in epilepsy group was significantly lower than that in the normal control group (P <0.01). The content of γ-aminobutyric acid in high and middle betaine groups was significantly higher than that in epilepsy group (P <0.01). The results of immunofluorescence and Western blotting showed that the expression of γ-aminobutyric acid receptor in epigallocatechin group was significantly higher than that in normal control group (P <0.01), betaine high, middle and low concentration group was significantly higher than epilepsy group (P <0.01); HE staining, sodium valproate group of liver tissue microscopy showed obvious vacuoles Degeneration (P <0.05). Conclusion Betaine has good anti-epileptic effect, and no hepatotoxicity.