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目的 探讨无针注射移植骨髓间充质干细胞(BMSCs)治疗缺血性心脏病的可行性。方法 健康杂种犬32只建立急性心梗(MI)模型并随机分为4组为单纯建模组（MI组），无针注射生理盐水组（NS组），有针注射移植干细胞组（N+MSCs组），无针注射移植干细胞组（NF+MSCs组）。术后6周检测血流动力学，处死动物后分离心脏行大体及组织学观察，计算MI面积并行新生血管计数。结果 N+MSCs组及NF+MSCs组梗死区心肌被纤维结缔组织替代，其间可见新生小血管；分别与MI组，NS组及N+MSCs组比较，NF+MSCs组左室舒张末压(9.00±3.2)mmHg(1 mmHg=0.133 kPa)下降、左室内压最大上升(841.86±72.85)mmHg/s和下降速率(-787.29±45.08)mmHg/s升高、梗死面积(18.59±3.58)%缩小、新生小血管数量(9.57±2.23)增多（P<0.05）。结论 无针注射移植干细胞治疗缺血性心脏病是安全，有效的。
Objective To investigate the feasibility of needle-free transplantation of bone marrow mesenchymal stem cells (BMSCs) for treatment of ischemic heart disease. Methods Thirty-two healthy beagle dogs were randomly divided into four groups: model group (MI group), needle-free saline group (NS group), needle-injected transplanted stem cell group MSCs group), needle-injected transplantation stem cell group (NF + MSCs group). Hemodynamics was measured at 6 weeks after operation. The hearts were removed and the heart was removed for gross and histological observation. The area of MI was calculated and the neovascular count was calculated. Results Compared with the MI group, the NS group and the N + MSCs group, the myocardium in the N + MSCs group and the NF + MSCs group was replaced by fibrous connective tissue with newborn small blood vessels. The left ventricular end diastolic pressure (n = (P <0.05). The increase of left ventricular pressure was the highest (841.86 ± 72.85) mmHg / s and the rate of descent was (-787.29 ± 45.08) mmHg / s and the infarct size was (18.59 ± 3.58)% , The number of newborn blood vessels (9.57 ± 2.23) increased (P & lt; 0.05). Conclusion The needle-free transplantation of stem cells for ischemic heart disease is safe and effective.