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应用免疫组织化学方法检测了42例原发性肝细胞癌（HCC）和癌旁肝细胞中p16的表达。结果显示，所有癌旁肝细胞p16表达均为阳性，18例癌细胞内p16蛋白阳性，阳性率为42．86％。p16蛋白弥漫性分布在肝细胞浆内，少数细胞核内也可见阳性显色。同一标本中癌旁细胞p16蛋白表达阳性，癌细胞内则可为阴性，表明用免疫组织化学方法在正常细胞中可检测到p16蛋白，而某些癌细胞可因p16缺失或突变等不能表达。p16表达还与HCC的组织学分级有关，分化程度高的HCCp16的表达率为66．67％（12／18），明显高于低分化HCC组（0／4），提示p16失活可能发生在HCC早期。
Immunohistochemical method was used to detect the expression of p16 in 42 cases of primary hepatocellular carcinoma (HCC) and adjacent liver cells. The results showed that p16 expression was positive in all paracancerous liver cells, and p16 protein was positive in 18 cancer cells. The positive rate was 42.86%. The p16 protein is diffusely distributed in the cytoplasm of liver cells, and positive staining is also seen in a few nuclei. The expression of p16 protein in paracancerous cells in the same specimen was positive, but it was negative in cancer cells, indicating that p16 protein can be detected in normal cells by immunohistochemistry. However, some cancer cells can not be expressed due to p16 deletion or mutation. P16 expression was also related to histological grade of HCC. The expression rate of HCCp16 with high degree of differentiation was 66.67% (12/18), which was significantly higher than that of low-differentiation HCC (0/4), suggesting that p16 inactivation may occur in Early HCC.