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目的:动态观察自体外周血造血干细胞移植患者经rhG-CSF动员过程中外周血及外周血造血干细胞(PBPC)中淋巴细胞亚群和CD34+细胞的变化,指导临床选择最佳采集时机。方法:对大剂量化疗(HDC)联合重组人粒细胞集落刺激因子(rhG-CSF)动员的38例白血病和淋巴瘤患者,采用流式细胞术(FACS)测定其动员前后外周血及PBPC中的淋巴细胞亚群CD3、CD4、CD8、NK、CD19和造血干细胞CD34细胞含量的变化,同时用甲基纤维素半固体培养基进行CFU-GM培养来评价干细胞克隆生成能力。结果:动员后外周血淋巴细胞亚群CD3、CD4细胞含量均低于动员前(P<0.01),而动员后外周血中的CD34细胞含量明显高于动员前(P(0.05)。动员后第5天CD34含量达到最高峰。PBPC中CD4、CD4/CD8明显低于外周血(P<0.005),其他淋巴细胞亚群含量与外周血比较无明显变化。动员后外周血中CD34+细胞明显高于动员后,动员后PBPC中CFU-GM生成明显高于外周血(P<0.05),CD4/CD8比值严重倒置,B细胞恢复较快。结论:大剂量化疗联合rhG-CSF动员会使外周血淋巴细胞亚群发生不同程度的变化并明显增加外周血造血干细胞含量。
OBJECTIVE: To dynamically observe the changes of lymphocyte subsets and CD34 + cells in peripheral blood and peripheral blood stem cells (PBPC) during mobilization of rhG-CSF in patients with autologous peripheral blood stem cell transplantation to guide the best timing of clinical selection. Methods: Thirty-eight patients with leukemia and lymphoma mobilized by high-dose chemotherapy combined with recombinant human granulocyte-colony stimulating factor (rhG-CSF) were measured by flow cytometry (FACS) before and after mobilization in peripheral blood and PBPC Lymphocyte subsets CD3, CD4, CD8, NK, CD19 and hematopoietic stem cells CD34 cell content changes, while using methylcellulose semi-solid medium for CFU-GM culture to evaluate stem cell clonogenic capacity. Results: After mobilization, the levels of CD3 and CD4 in peripheral blood lymphocyte subsets were lower than those before mobilization (P <0.01), but the levels of CD34 in peripheral blood were significantly higher than those before mobilization (P 0.05) 5 days CD34 content reached its peak .PBPC CD4, CD4 / CD8 was significantly lower than the peripheral blood (P <0.005), other lymphocyte subsets compared with peripheral blood no significant changes in peripheral blood mobilized CD34 + cells was significantly higher After mobilization, mobilization of CFU-GM in PBPC was significantly higher than that in peripheral blood (P <0.05), the ratio of CD4 / CD8 was seriously inverted, and the recovery of B cells was faster.Conclusion: High-dose chemotherapy combined with rhG-CSF mobilization can cause peripheral blood lymph Cell subpopulations changed to varying degrees and significantly increased peripheral blood hematopoietic stem cell content.