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目的通过检测安徽汉族人中急性心肌梗死(AMI)患者及健康对照者的血小板膜糖蛋白(GP)受体Ⅰa C807T基因多态性,明确血小板GPⅠa C807T的基因型,探讨其多态性与AMI之间的关系。方法提取人基因组DNA,用多聚酶链反应(PCR)扩增AMI组(74例)和健康对照组(60例)外周血DNA的基因片段,用琼脂糖凝胶分离扩增产物,在紫外透射仪下观察结果并分析。结果血小板膜糖蛋白Ⅰa-807基因多态性分析:AMI组的GPⅠa807TT+TC基因型携带者频率显著高于对照组。结论Ⅰa 807TT+TC的高表达是AMI患者的重要危险指标,Ⅰa C807T多态性可能与AMI的发生密切相关。
Objective To detect the gene polymorphism of platelet glycoprotein (GP) receptor Ⅰ a C807T in patients with acute myocardial infarction (AMI) and healthy controls in Anhui Han population, to determine the genotype of platelet GP Ⅰ a C807T and to explore its association with AMI The relationship between. Methods Human genomic DNA was extracted and the DNA fragments of peripheral blood were amplified by polymerase chain reaction (PCR) from AMI group (74 cases) and healthy control group (60 cases). The amplified products were separated by agarose gel and analyzed by UV - Under the observation and analysis. Results The platelet membrane glycoprotein Ⅰ a-807 gene polymorphism analysis: AMI group GPⅠa807TT + TC genotype frequency was significantly higher than the control group. Conclusion The high expression of Ia 807TT + TC is an important risk index for patients with AMI. The Ia C807T polymorphism may be closely related to the occurrence of AMI.