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已经发表过几篇讨论核多角体病毒预处理和染色的方法。A.Huger(昆虫病理学杂志。3,338—341,1961),J.J.Hamm(无脊推动物病理学杂志。8,125—126,1966),和J.Weiser及N.W.Hussey[在《昆虫和螨类的微生物防治》(H.D.Burges和N.W.Hus-sey.主编)pp.13-66,纽约出版,1971]都列出了需要很长的预处理和较长的染色过程的方法。即使是标准的吉姆沙(Giemsa)技术剂量,如果不经复杂的初染处理也不能使包含体着色。我们已经找到了使用一种很强的、并且可以长久保存的酸性染色剂一偶氮胭脂红G(az-
Several articles have been published on methods for pretreatment and staining of nuclear polyhedrosis virus. A. Huger, J. Entomology. 3, 338-341, 1961), JJ Hamm (Journal of Vertebrate Pusher Pathology. 8, 125-126, 1966), and J. Weiser and NW Hussey [in “ Prevention and Treatment ”(Eds. HD Burges and NWHus-sey) pp. 13-66, New York, 1971, both list methods that require long pretreatment and longer staining processes. Even at standard Giemsa technical dosages, occlusion bodies can not be occluded without complex initial dye treatment. We have found the use of a strong, long-lasting acid stain azo carmine G (az-