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作者从尾蚴感染的小鼠分别获得曼氏血吸虫和日本血吸虫的雌雄合抱成虫,从金色仓鼠获得埃及血吸虫成对成虫,放在冰上使雌雄分离,用[~(35)S]蛋氨酸对雌雄成虫糖蛋白作标记,再经Con-A琼脂糖亲和层析柱进行分离,将糖蛋白在冰上与金黄色葡萄球菌共同孵育15分钟,以减少非特异性的结合。聚丙稀酰胺凝胶电泳和荧光X线照像系按Strand等(1982)的方法进行。检测的血清有曼氏血吸虫病、埃及血吸虫病、混合感染日本血吸虫病和旋毛虫病、猪囊尾蚴病、细粒棘球蚴病、溶组织内阿米巴病、班氏丝虫病等病人的血清及正常人的血清。
The authors obtained male and female adult worms of Schistosoma mansoni and Schistosoma japonicum respectively from mice infected with cercariae, obtained pairs of adults of Schistosoma japonicum from golden hamsters and separated them from each other on ice, using [~ (35) S] methionine for both male and female adults Glycoproteins were labeled and separated on a Con-A agarose affinity column. Glycoproteins were incubated with Staphylococcus aureus for 15 minutes on ice to reduce nonspecific binding. Polyacrylamide gel electrophoresis and fluorescent X-ray images were performed as described by Strand et al. (1982). Serum were detected in Schistosoma mansoni, schistosomiasis japonica, mixed infection of schistosomiasis japonica and trichinosis, cysticercosis, echinococcus granulosus, Entamoeba histolytica, Bancroftian filariasis and other patients Of serum and normal human serum.