论文部分内容阅读
自烛缸法成功地培养恶性疟原虫(Pf)后,间日疟原虫(Pv)的体外培养却屡遭失败。作者认为对某些值得仔细考虑的理论问题的研究,可能有助于Pv体外培养技术的建立,并认为在培养任何一种原虫时,均须仔细研究宿主与寄生虫的关系。Pf的滋养体和裂殖体系在内脏毛细血管和窦状隙内发育成熟的,而Pv则在周围血及内脏内发育,表明这两种原虫发育所需的二氧化碳和氧压要求不一。Pf和Pv的生理特点有所不同,寄生Pf的红细胞缩小,而寄生Pv的则胀大,Pf的染色质和胞浆少于Pv,Pv的红内期发育时间长于Pf。因此Pv所需的能量酶和氨基酸可能多于Pf,这也可能是Pv体外培养失败的原因。据此认为,烛缸法不适于PV的培养,建议研制可交替充以氮和二氧化碳以及氮和氧的混合气体系的培养系,从而改变氧和二氧化碳的含量,以模拟人体动静脉内红细胞所
After P. falciparum (Pf) was successfully cultured by candle jar method, in vitro culture of P. vivax (Pv) failed repeatedly. The authors believe that some of the theoretical issues that warrant careful consideration may contribute to the establishment of Pv in vitro culture techniques and suggest that the host-parasite relationship should be carefully studied in the cultivation of any protozoan. The trophozoites and fission systems of Pf mature in the visceral capillaries and sinusoids while Pv develops in the peripheral blood and viscera, suggesting different requirements for the development of these two protozoa. Pf and Pv physiological characteristics are different, parasitic Pf red blood cells shrink, while the parasitic Pv is swollen, Pf chromatin and cytoplasm less than Pv, Pv red-phase development longer than Pf. Therefore Pv energy enzymes and amino acids may be more than Pf, which may also be the reason for the failure of Pv in vitro culture. Therefore, the candle cylinder method is not suitable for the cultivation of PV, it is proposed to develop a culture system that can be alternately filled with a mixture of nitrogen and carbon dioxide and nitrogen and oxygen to change the oxygen and carbon dioxide content to simulate the human arterial and venous red blood cells