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为了揭示不同生态区烤烟风格特色形成的机理,采用Agilent烟草寡聚芯片技术,对商洛市洛南县和宝鸡市陇县(对照)两个生态区烤烟打顶后15 d同一品种(秦烟96)中部叶的基因表达谱进行了初步分析。结果表明:与陇县相比,在44 000个基因中洛南烟区烟叶有2 058个基因差异表达,其中上调基因962个,占46.74%;下调基因1 096个,占53.26%。按功能富集分类,这些差异基因主要富集在代谢、响应外界刺激及生理调控等生物学过程,大部分位于胞外区与细胞内膜系统。其中洛南烟区碳水化合物合成代谢较为活跃,陇县烟区萜类化合物代谢、苯丙烷代谢、芳香族化合物合成代谢较为活跃。基因代谢通路分析表明,细胞信号转导、转录因子及参与植物非生物胁迫相关基因在两个烟草种植区均为高表达。
In order to reveal the formation mechanism of flue-cured tobacco in different ecological zones, Agilent’s tobacco oligomer chip technology was used to analyze the characteristics of flue-cured tobacco in Luonan County of Shangluo City and Long County of Baoji City (control) ) Middle leaf gene expression profile of a preliminary analysis. The results showed that compared with Longxian County, there were 2 058 genes in Luonan tobacco growing in 44 000 genes, of which 962 genes were up-regulated, accounting for 46.74%, and 1 096 genes were down-regulated, accounting for 53.26%. By functional enrichment classification, these differential genes are mainly concentrated in the biological processes of metabolism, response to external stimuli and physiological regulation, most of which are located in the extracellular and intracellular membrane systems. Among them, the synthesis and metabolism of carbohydrates in Luonan tobacco area were relatively active. The metabolism of terpenoids, the metabolism of phenylpropane and the anabolic activities of aromatic compounds in Longxian tobacco area were relatively active. Gene metabolic pathway analysis showed that cell signal transduction, transcription factors and genes involved in plant abiotic stress were highly expressed in both tobacco growing areas.