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从鲨鱼肝中分离纯化了鲨鱼肝刺激物质(sHSS),并对其理化性质进行了研究.纯化过程包括匀浆、离心、超滤、DEAE-sephadex A25层析、FPLC mono Q层析.经此过程,sHSS活性可提高到9000IU/mg,纯化倍数可达750倍.经SDS聚丙烯酰胺凝胶电泳和高效凝胶过滤检测分别只出现一条带和一个洗脱峰.用质谱法测其分子量为16973Da.用PE-ABD491A蛋白质N-末端测序仪测定其N-末端氨基酸排列顺序是N-Met-Arg-Thr-Gln-Glu-His-Thr-Lys-Ser-Val.sHSS具有热稳定性和耐酸碱性.“,”A hepatic stimulator substance from Shark liver (sHSS) was purified and characterized. The purification procedure included homogenization, centrifugation, ultrafiltration, DEAE-sephadex A25 chromatography and FPLC mono Q chromatography. By the procedure, the stimulatory specific activity of sHSS could be increased to 9000IU/mg, and was 750 times more than the crude extract. The purity of the final purified fraction F70 which retained the maximal activity of sHSS was checked by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) and High Performance Gel Filtration respectively, and showed only one band or one peak. Its molecular weight was measured to be 16973Da by mass spectrometry. Its N-terminal sequence was determined to be N-Met-Arg-Thr-Gln-Glu-His-Thr-Lys-Ser-Val by PE-ABD 491A Protein N-Terminal Sequencing Meter. It was stable over a wide range of pH and temperature.