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目的:分析3个T细胞亚群,CD4+T、CD8+T和调节性T(regulatory T,Treg)细胞在胰腺癌病人外周血中的变化,为胰腺癌免疫治疗中靶细胞的确认提供理论依据。方法:通过免疫荧光抗体标记和流式细胞检测外周血中CD4+T、CD8+T和Treg细胞,分析胰腺癌病人外周血中CD4+T、CD8+T和Treg细胞的水平与胰腺癌临床病理特征的相关性。结果:胰腺癌病人外周血中CD4+T、CD8+T的水平与对照组相比无统计学差异,而Treg细胞在CD4+T细胞中的比例较对照组显著升高;除了Treg的比例与胰腺癌的疾病分期密切相关外,3个细胞亚群的水平与胰腺癌病人临床病理特征无相关性。胰腺癌合并梗阻性黄疸行胆道支架引流术后,CD4+T、CD8+T细胞的水平无变化,但Treg细胞的比例显著下降。结论:在胰腺癌病人的免疫功能状态的变化中Treg细胞介导的免疫抑制可能占主导地位。合并梗阻性黄疸行胆道引流术可缓解Treg细胞介导的免疫抑制。
OBJECTIVE: To analyze the changes of three T cell subsets, CD4 + T, CD8 + T and regulatory T (Treg) cells in the peripheral blood of patients with pancreatic cancer so as to provide theoretical basis for the confirmation of target cells in the immunotherapy of pancreatic cancer in accordance with. Methods: Peripheral blood CD4 + T, CD8 + T and Treg cells were detected by immunofluorescence antibody and flow cytometry. The levels of CD4 + T, CD8 + T and Treg cells in peripheral blood of patients with pancreatic cancer were analyzed. Correlation of features. Results: There was no significant difference in the levels of CD4 + T and CD8 + T in peripheral blood of patients with pancreatic cancer compared with the control group, while the proportion of Treg cells in CD4 + T cells was significantly higher than that in the control group. Pancreatic cancer is closely related to the stage of the disease, the level of three cell subsets and pancreatic cancer patients with no correlation between clinicopathological features. In pancreatic cancer with obstructive jaundice, the levels of CD4 + T and CD8 + T cells did not change after biliary stenting, but the proportion of Treg cells was significantly decreased. CONCLUSIONS: Treg cell-mediated immunosuppression may predominate in changes in immune status in patients with pancreatic cancer. Bile duct drainage with obstructive jaundice can relieve Treg cell-mediated immunosuppression.