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过氧化物酶V(peroxiredoxin V,Prx V)是过氧化物酶(peroxiredoxin)家族中的一员,广泛存在于线粒体、溶酶体及细胞质中,具有有效地清除细胞内一氧化氮(nitric oxide,NO)和活性氧(reactive oxygen species,ROS)的功能。该文阐明β-拉帕醌(β-lapachone)引起SW480结肠癌细胞凋亡过程中Prx V的作用及其机制。该研究利用β-拉帕醌处理SW480细胞系,通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT]法、荧光显微镜照相、流式细胞术、蛋白质免疫印迹分析等方法检测细胞存活率、细胞内ROS水平、细胞凋亡情况以及凋亡相关蛋白质水平的变化。结果表明,β-拉帕醌通过抑制Prx V水平,致使细胞内ROS水平升高,激活线粒体依赖性凋亡途径,导致SW480结肠癌细胞凋亡。研究结果初步揭示了β-拉帕醌诱发SW480结肠癌细胞发生凋亡的机制,为结肠癌的治疗提供了新思路。
Peroxiredoxin V (PrxV) is a member of the peroxiredoxin family, widely present in mitochondria, lysosomes and cytoplasm. It has the ability to effectively eliminate nitric oxide , NO) and reactive oxygen species (ROS) function. This article illustrates the role and mechanism of Prx V during β-lapachone-induced apoptosis in SW480 colon cancer cells. In this study, SW480 cell lines were treated with [beta] -papaquinone and treated with 3- (4,5-dimethyl-2,5-diphenyltetrazolium bromide 2-thiazolyl) -2,5-diphenyl-2-H-tetrazolium bromide (MTT) method was used to detect the cell viability, intracellular ROS level and cell apoptosis by fluorescence microscopy, flow cytometry and Western blot analysis Death and apoptosis-related protein levels. The results showed that β-lapachone induced the apoptosis of SW480 colon cancer cells by inhibiting the Prx V level, leading to the increase of intracellular ROS levels and activation of mitochondria-dependent apoptotic pathway. The preliminary results revealed that β-lapachone induced apoptosis in SW480 colon cancer cells and provided a new idea for the treatment of colon cancer.