论文部分内容阅读
败血症导致多器官衰竭(MOF)的发病机制与多形粒细胞(PMN)功能失学以及补体激活异常有关.作者选择11例败血症病人确诊MOF者为Ⅰ组;3例败血症病人无MOF者为Ⅱ组;3例手术病人无明显感染者为Ⅲ组;再以3例健康人为对照组.此20例均采静脉血标本分离出PMN作研究.(一)PMN—内皮细胞粘附测定:按Gimbrone等介绍的方法备制健康人体新鲜脐带静脉的内皮细胞(HUVEC)用相衬显微镜(phase-contrast micro-scope)观察PMN粘附到内皮细胞的数目,以此来估计PMN表面淋巴细胞功能相关抗原—1(LFA—1)即
The pathogenesis of sepsis-induced multiple organ failure (MOF) is associated with dysfunction of polymorphonuclear leukocytes (PMNs) and abnormal complement activation. The authors selected 11 patients with sepsis who were diagnosed with MOF as Group I, 3 patients with sepsis without MOF as Group II ; 3 cases of surgical patients without obvious infection group Ⅲ; then 3 healthy people as the control group.These 20 cases were collected from venous blood samples were isolated PMN (a) PMN-endothelial cell adhesion assay: According to Gimbrone et al Introduction The preparation method of fresh umbilical vein endothelial cells (HUVEC) of healthy human is used to observe the number of PMN adhering to endothelial cells by phase-contrast micro-scope to estimate the expression of PMN surface lymphocyte function- 1 (LFA-1) that is