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目的 探讨老年妇女外阴外阴营养不良组织中表皮生长因子受体 (EGFR)及p5 3表达及临床意义。方法 应用免疫组化法对老年妇女外阴正常外阴皮肤、慢性外阴营养不良、不典型增生和外阴癌组织进行标记。结果 EGFR在正常外阴皮肤基底层有弱阳性表达 ,p5 3均阴性。EGFR在增生型、硬化苔癣型和混合型营养不良三者之间阳性率比较 ,差异均无显著性意义 (P >0 .0 5 ) ;不典型增生和鳞癌的阳性率均明显高于各型营养不良 (P <0 .0 5 ) ;而鳞癌与不典型增生之间差异无显著性意义 (P >0 .0 5 )。p5 3基因蛋白在外阴不典型增生和外阴癌的表达率显著高于外阴营养不良病变的各型营养不良组织 (P <0 .0 1)。结论 老年妇女外阴癌组织中p5 3与EGFR阳性表达率较高 ,EGFR与p5 3突变在老年妇女外阴癌的发生过程中起重要作用。
Objective To investigate the expression of epidermal growth factor receptor (EGFR) and p5 3 in vulvar and vulvar dystrophy tissues in elderly women and its clinical significance. Methods The normal vulva skin, chronic vulvar dystrophy, dysplasia and vulval cancer tissues of elderly women were labeled by immunohistochemistry. Results EGFR had a weak positive expression in the basal layer of normal vulva, but all of p5 3 were negative. The positive rates of EGFR in proliferative, lichen planus and mixed dystrophy were not significantly different (P> 0.05); the positive rates of atypical hyperplasia and squamous cell carcinoma were significantly higher than those of All types of malnutrition (P <0. 05); and squamous cell carcinoma and atypical hyperplasia no significant difference (P> 0.05). The expression of p5 3 protein in vulvar atypical hyperplasia and vulvar cancer was significantly higher than that in dystrophic vulvar dysplasia (P <0.01). Conclusion The positive rate of p5 3 and EGFR in vulvar cancer tissues is higher in elderly women, and EGFR and p5 3 mutations play an important role in the pathogenesis of vulvar cancer in elderly women.