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利用来航鸡胚,于孵育第3d,从气室注入0.2ml/卵AlCl_3水溶液(1.708μmol/卵)。取给药组与对照组第4d和第6d神经管及第6d以后小脑,在光镜及电镜下观察;并用~3H-TdR或~3H-methionine标记。发现鸡胚神经管形成后,生发层细胞的~3H-TdR标记率以翼板区最高,其次是基板区;给药组标记率低于对照组;神经管生发层细胞有丝分裂相减少;套层细胞的尼氏体(光镜下)、粗面内质网和游离核蛋白体(电镜下)减少。
Using Broiler embryos, on the 3rd day of incubation, 0.2 ml/water solution of egg AlCl_3 (1.708 μmol/egg) was injected from the air chamber. The cerebellum of the administration group and the control group on the 4th and 6th day, and the cerebellum on the 6th day, were observed under the light microscope and the electron microscope, and labeled with ~3H-TdR or ~3H-methionine. After the formation of chicken embryonic neural tube, the labeling rate of ~3H-TdR in germinal layer cells was the highest in the wing area, followed by the substrate area; the labeling rate in the administration group was lower than that in the control group; the mitotic phase in the neural tube layer was reduced; The cells were reduced in Nissl (light microscopy), rough endoplasmic reticulum, and free ribosome (under electron microscopy).