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目的:建立苯并芘诱导的小鼠腹主动脉瘤模型,并与经典ApoE基因敲除(ApoE~(-/-))模型进行比较。方法:采用苯并芘高脂饲料喂养C57BL/6小鼠合并皮下注射血管紧张素II(Bap模型)诱导腹主动脉瘤的形成,造模第28天与ApoE~(-/-)模型比较腹主动脉瘤形成率、大体观的差别以及组织形态学的不同。结果:与正常对照组比较,苯并芘模型实验组小鼠腹主动脉表现为管腔扩张,直径增大(P<0.001)且未出现壁间血肿,成瘤率为40%;ApoE~(-/-)模型实验组小鼠腹主动脉直径明显增加(P<0.01)并伴有壁间血肿形成,成瘤率为60%。结论:与ApoE~(-/-)模型比较,苯并芘模型成瘤率虽然有所降低,但造模成本低廉同时模拟人类主动脉瘤的病理特征。
OBJECTIVE: To establish a model of abdominal aortic aneurysm induced by benzopyrene in mice and compare with classical ApoE knockout (ApoE ~ (-) -) model. Methods: C57BL / 6 mice were fed with benzopyrene and high fat diet to induce the formation of abdominal aortic aneurysm by subcutaneous injection of angiotensin II (BAP model). On the 28th day, the abdominal aorta aneurysms were compared with the ApoE ~ (- / -) model Aortic aneurysm formation rate, the general difference and histological differences. Results: Compared with the normal control group, the abdominal aorta in the experimental group showed luminal dilatation and diameter increase (P <0.001), and no hematoma occurred on the wall. The tumorigenic rate was 40% - / -) Model The abdominal aorta diameter increased significantly (P <0.01) and accompanied by intramural hematoma formation, the rate of tumor formation was 60%. Conclusion: Compared with the ApoE ~ (- / -) model, the tumorigenic rate of benzopyrene model is reduced, but the cost of modeling is low and the pathological features of human aortic aneurysm are simulated at the same time.