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目的制备基于壳聚糖-碳酸钙-纳米金复合膜固定乙酰胆碱酯酶的新型电化学传感器。方法一步电沉积壳聚糖、碳酸钙和纳米金修饰到玻碳电极,由于电沉积过程有气泡产生,使沉积到电极上的碳酸钙微粒形成了三维多孔的结构,利于酶比较分散的固定在电极表面,提高检测效率;同时纳米金的引入不仅增大了电极比表面积,而且促进电子的快速传递,放大了检测信号。结果将制备的生物传感器用来检测有机磷农药乐果,检测限为2.5 pg/mL,检测时间只需要7 min。结论该生物传感器检测法实现了对有机磷农药快速、超灵敏的检测。
Objective To prepare a novel electrochemical sensor based on chitosan-calcium carbonate-gold nanoparticle composite immobilization of acetylcholinesterase. Methods Electrodeposition of chitosan, calcium carbonate and nano-gold modified to glassy carbon electrode, as a result of bubble generation electrodeposition process, so that deposited on the electrode calcium carbonate particles formed a three-dimensional porous structure, which will help the enzyme more dispersed in the fixed Electrode surface to improve the detection efficiency; at the same time the introduction of nano-gold not only increases the specific surface area of the electrode, but also promotes the rapid transfer of electrons and amplifies the detection signal. Results The prepared biosensor was used to detect organophosphorus pesticide dimethoate with a detection limit of 2.5 pg / mL and a detection time of only 7 min. Conclusion The biosensor detection method to achieve rapid and ultra-sensitive detection of organophosphorus pesticides.