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１材料和方法１．１材料 大肠杆菌Ｋ８０２、人胚肾细胞２９３由本室验室保存。细胞转染试剂脂质体Ｃｌｏｎｆｅｃｔｉｎ购自Ｃｌｏｎｔｅｃｈ公司。ＤＭＥＭ购自ＧＩＢ－ＣＯ公司。Ｇ４１８购自Ｓｉｇｍａ公司。胎牛血清购自杭州四季青公司。鼠抗人ＴＨＡＮＫ单克隆抗体由倪?
1 Materials and Methods 1.1 Materials E. coli K802, 293 human embryonic kidney cells stored by the laboratory room. Clonfectin, a cell transfection reagent, was purchased from Clontech. DMEM was purchased from GIB-CO. G418 was purchased from Sigma. Fetal calf serum from Hangzhou Evergreen company. Mouse anti-human THANK monoclonal antibody by Ni?