论文部分内容阅读
为探讨鹿蹄草素对苹果轮纹病菌的抑制活性及作用机理,采用倍比稀释法测定鹿蹄草素最小抑菌浓度(MIC)和最小杀菌浓度(MBC),扫描和透射电镜观察鹿蹄草素对苹果轮纹病菌超微结构的影响。结果表明,鹿蹄草素对苹果轮纹病菌具有较强的抑菌活性,其最小抑菌质量浓度为0.078 mg/mL,最小杀菌质量浓度为0.156mg/mL。用扫描电镜观察,菌丝扭曲变形,菌丝体之间相互粘连,细胞壁破裂,表面出现絮状凝集物,部分菌丝断裂、消融、干瘪、空腔。用透射电镜观察,细胞壁明显变薄,胞内空腔增多,细胞内部组成紊乱,原生质大量外泄,细胞结构遭到严重破坏,最终菌体崩解死亡。
In order to investigate the inhibitory activity and the mechanism of the components of Pyrenophylla on apple rings rot pathogen, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of pilosifutal were determined by fold dilution method. Effect of Hypocrellin on the Ultrastructure of Apple Rot Disease. The results showed that the genus Pygidurin had strong antibacterial activities against the pathogen of apple rings, the minimum inhibitory concentration was 0.078 mg / mL and the minimum bactericidal concentration was 0.156 mg / mL. Scanning electron microscopy showed that the mycelium was distorted, the mycelium adhered to each other, the cell wall ruptured, the flocculent aggregates appeared on the surface, and some mycelium were broken, ablated, dry and hollow. Transmission electron microscopy showed that the cell wall was significantly thinned, the intracellular cavity was increased, the internal cellular components were disorganized, the protoplasm was excreted in a large amount, the cell structure was severely damaged, and finally the cells collapsed and died.