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目的:检测1型糖尿病(T1DM)、2型糖尿病(T2DM)、2型糖尿病伴动脉硬化(DAS)患者外周血白细胞端粒长度,分析糖尿病患者端粒长度变化的因素。方法:选择T1DM患者30例、T2DM患者60例、DAS患者40例和健康对照(NC组)40例,分别提取外周血白细胞,然后提取基因组DNA进行real-time PCR检测端粒长度。利用多元线性回归分析影响端粒长度变化的因素。结果:T1DM组、T2DM组和DAS组的端粒长度均小于NC组(P<0.05);T1DM组端粒长度短于T2DM组和DAS组;DAS组较T2DM组更短。多元线性回归分析显示,T1DM组中,年龄与端粒长度呈负相关(P<0.05);T2DM组中,年龄、体重指数(BMI)与端粒长度呈负相关(P<0.05);DAS组中,患病时间、BMI与端粒长度呈负相关(P<0.05)。结论:糖尿病患者的外周血白细胞端粒长度明显短于正常人,并且T1DM患者端粒长度短于T2DM;在2型糖尿病的对比中,DAS患者的端粒长度明显短于T2DM。患者的年龄、患病时间、BMI与端粒长度的缩短有密切的关系。
Objective: To detect telomere length of peripheral blood leukocytes in type 1 diabetes mellitus (T2DM), type 2 diabetes mellitus (T2DM) and type 2 diabetic patients with atherosclerosis (DAS) and to analyze the changes of telomere length in diabetic patients. Methods: Thirty patients with T1DM, 60 patients with T2DM, 40 patients with DAS and 40 healthy controls were enrolled in this study. Peripheral blood leucocytes were isolated and genomic DNA was extracted for telomere length detection. Factors influencing telomere length change were analyzed by multivariate linear regression. Results: The length of telomere in T1DM group, T2DM group and DAS group were less than that in NC group (P <0.05). The length of telomere in T1DM group was shorter than that in T2DM group and DAS group. DAS group was shorter than T2DM group. Multivariate linear regression analysis showed that there was a negative correlation between age and telomere length in T1DM group (P <0.05). There was a negative correlation between age and body mass index (BMI) and telomere length in T2DM group (P <0.05) During the diseased time, BMI was negatively correlated with telomere length (P <0.05). Conclusion: The telomere length of peripheral blood leucocytes in diabetic patients is significantly shorter than that in normal subjects, and the telomere length of T1DM patients is shorter than that of T2DM patients. In type 2 diabetes patients, the telomere length of DAS patients is significantly shorter than that of T2DM patients. The patient’s age, prevalence, BMI and telomere length are closely related.