【摘 要】
：
A non-invasive detection method for the status analysis of cell culture is presented based on digital holography technology. Lensless Fourier transform digital holography (LFTDH) configuration is developed for living cell imaging without prestaining. Comp
【机 构】
：
CollegeofAppliedSciences,BeijingUniversityofTechnology,Beijing100124,ChinaCollegeofLifeScienceandBio
【出 处】
：
ChineseOpticsLetters
论文部分内容阅读
A non-invasive detection method for the status analysis of cell culture is presented based on digital holography technology. Lensless Fourier transform digital holography (LFTDH) configuration is developed for living cell imaging without prestaining. Complex amplitude information is reconstructed by a single inverse fast Fourier transform, and the phase aberration is corrected through the two-step phase subtraction method. The image segmentation is then applied to the automatic evaluation of confluency. Finally, the cervical cancer cell TZMbl is employed for experimental validation, and the results demonstrate that LFTDH imaging with the corresponding image post-processing can provide an automatic and non-invasive approach for monitoring living cell culture.
其他文献
本文介绍了光学玻璃条纹气泡的全息干涉检验.与以往的检验方法相比,全息干涉法具有测量精度高和易于实现检验工作的自动化等特点.
对运行速度高达每分钟2000英尺的髙速过程进行质量控制的测量可能是很困难的。在这样高的速度下无法进行接触式测量，因而要停下生产线来进行定期检验。如果在产品检验时发现由于模具磨损或其它某种问题使产品超差，一整批产品就得废弃或返工。
Gain saturation is a significant phenomenon of fiber Raman amplifiers (FRAs). Gain figures versus signal power are well explained. For the small signal, the coupled ordinary differential equations are used, and for the large signal, the Raman gain coeffic
针对隧道施工中双护盾硬岩隧道掘进机位姿的精密测量需求,设计了一种组合式位姿测量系统。系统把单目视觉和激光标靶相结合,在利用激光标靶和全站仪完成掘进机后盾体位姿测量基础上,通过所设计的光学特征点空间分布模型,实现特征点的准确识别,根据特征点之间已知的几何约束关系和摄像机的透视投影模型,进行实时的图像处理和位姿解算,实现掘进机前盾体相对后盾体位姿的非接触式测量。系统建立了光学特征点、智能视觉传感器、激
报道了双端抽运连续输出的掺Yb3 双包层高功率光纤激光器。实验采用了中心波长在975 nm附近的两种输出形式的半导体激光器(LD)作为抽运源,测量了不同抽运条件下的输出功率特性和光谱特性。在仅尾纤输出的半导体激光器抽运下获得了斜率效率为42%,峰值波长为1103.8 nm的9.2 W激光输出;在仅准直输出的半导体激光器抽运下获得了斜率效率为57%,峰值波长为1104.4 nm的20.0 W激光输出;当两个半导体激光器在双端同时抽运时,获得光纤激光最大输出功率为30.6 W,输出峰值波长为1108.4 nm
根据行星夹具的膜厚理论模型, 对半球透镜表面的膜厚分布进行理论分析研究。推算半球面上典型点的膜厚公式, 用代 入法对不同情况的膜厚分布进行计算, 结果表明半球面上中心区域(90°张角内)的膜厚不均匀性可控制在10%左右, 其边缘区 域(张角大于90°)的膜厚不均匀性高达90%; 改变蒸发源的位置对改善膜厚均匀性没有明显帮助, 但改变蒸发源的形式能改善 膜厚均匀性。该研究工作对半球透镜表面镀膜的膜厚均匀性分析及改善具有一定的理论指导意义。
本文介绍利用ATR方法观察LB膜的聚合过程,研究测量聚合前后LB膜光学性质的变化,所得的结果和化学键理论值相符合。在聚合过程中ATR谱的变化相当明显,首次观察到有机单分子在聚合过程中直立起来。
Optical-resolution photoacoustic microscopy (OR-PAM) has been shown to be an excellent tool for high-resolution imaging of microvasculature, and quantitative analysis of the microvasculature can provide valuable information for the early diagnosis and tre
We investigate InAs/GaAs quantum dot (QD) lasers grown by gas source molecular beam epitaxy with different growth temperatures for InAs dot layers. The same laser structures are grown, but the growth temperatures of InAs dot layers are set as 425 and 500