用反相高效液相法，对血浆非那西汀药物浓度的测定方法及其药代动力学进行了研究。实验采用Ｗａｔｅｒｓ公司ＨＰＬＣ系统，国产ＺＯＲＲＡＸＢＰＣＮ柱，以磷酸盐缓冲液／乙氰为流动相，内标法定量。本方法标准血浆线性关系良好（ｒ＝０９９９９），线性范围０７０～８９２８μｍｏｌ／Ｌ，最低检测量为１２５ｎｇ，平均方法回收率为９９９２％，浓度为８９２８μｍｏｌ／Ｌ、１１１６μｍｏｌ／Ｌ的提取回收率分别为９４８％、８３１％和８２４％；精密度考察批内变异系数（ＣＶ％）分别为０４９、３６５和４９０，批间变异系数（ＣＶ％）分别为３９３、３６４和５９３。 RP-HPLC method was used to determine the concentration of phenacetin in plasma and its pharmacokinetics. Experiments using Waters HPLC system, made ZORRAX BP column, with phosphate buffer / acetonitrile as the mobile phase, internal standard method. The standard linearity of this method was good (r = 0.9999) with a linear range of 070-8928μmol / L, with the lowest detection level of 125ng. The average recovery was 9992% with a concentration of 8928μmol / L and 1116μmol / L were 948%, 831% and 824%, respectively. The coefficient of variation (CV%) within the precision was 0.49, 3.65 and 490, the inter-assay coefficient of variation (CV%) were 393,364 and 593, respectively.