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通过高效液相反相色谱、毛细管反相色谱 -串联质谱联用方法对SARS病毒攻击细胞的研究 ,鉴定出了N、S和M 3种SARS结构蛋白质 ,并准确测定了N蛋白质的分子量。由分子量结果和生物信息学推断的N蛋白质的理论分子量比较 ,可以确定N蛋白质不存在常见的磷酸化修饰和糖基化修饰或含量很低。进一步的研究表明 :N蛋白质可能存在降解现象 ,其降解机理尚需探索。另外 ,通过对样品直接酶切后进行两维毛细管分离和串联质谱鉴定与对样品先进行分离 ,然后再进行毛细管反相分离 串联质谱鉴定方法比较 ,表明两种方法在蛋白质组学研究中各具优缺点 ,可根据不同目的选择使用
The SARS virus-challenged cells were studied by high performance liquid chromatography and capillary chromatography-tandem mass spectrometry. N, S and M SARS structural proteins were identified, and the molecular weight of N protein was accurately determined. A comparison of the theoretical molecular weights of N proteins deduced from molecular weight results and bioinformatics confirms that there is no common phosphorylation and glycosylation modification or low content of N proteins. Further studies have shown that: N protein may exist degradation phenomenon, the mechanism of its degradation still need to be explored. In addition, the direct digestion of samples by two-dimensional capillary separation and tandem mass spectrometry identification and separation of samples prior to capillary reverse phase separation tandem mass spectrometry identification method comparison, indicating that the two methods in the proteomics study Advantages and disadvantages, according to different purposes choose to use