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为研究灰树花发酵产物提取出的胞内多糖的免疫活性,采用50只雄性昆明小鼠,随机分为5组,每组10只,设100、200和400mg/(kg·d)3个水平处理组,并设阳性对照组(黄芪多糖400mg/(kg·d))和阴性对照组(生理盐水),饲养30d后观察多糖对细胞免疫功能的影响。通过流式细胞仪检测脾细胞表面分子的CD4和CD8及对T淋巴细胞增殖的影响;用实时荧光定量PCR(RT-PCR)检测其细胞因子IL-6、IL-8、CRP和TNF-α的表达。结果表明:灰树花胞内多糖使小鼠脏器显著增大(P≤0.05);并显著增加了脾淋巴细胞的增殖能力(P≤0.05);对于T淋巴细胞亚群中免疫细胞CD4/CD8的比值随着用药量的增加而提升(P≤0.05);同时小鼠肠道中的细胞因子IL-6、IL-8和TNF-α的分子表达量起到了上调作用(P≤0.05);并且下调了炎症因子CRP的分子表达量(P≤0.05)。灰树花菌丝体胞内多糖能使小鼠体内免疫器官增大,免疫细胞分泌增多,相关免疫因子表达量增加。
In order to study the immunological activity of polysaccharides extracted from Grifola frondosa fermentation products, 50 male Kunming mice were randomly divided into 5 groups (n = 10, 100, 200 and 400 mg / (kg · d) (APS 400mg / (kg · d)) and negative control group (NS). The effects of polysaccharide on cellular immune function were observed after 30 days of rearing. The effects of CD4 and CD8 on the surface of spleen cells and the proliferation of T lymphocytes were detected by flow cytometry. The levels of cytokines IL-6, IL-8, CRP and TNF-α were detected by real-time fluorescence quantitative PCR expression. The results showed that Grifola frondosa polysaccharide significantly increased the number of organs in mice (P≤0.05), and significantly increased the proliferation of splenic lymphocytes (P≤0.05). The percentage of CD4 / (P≤0.05). Meanwhile, the molecular expressions of IL-6, IL-8 and TNF-α in the intestine of mice were up-regulated (P≤0.05). And down-regulated the molecular expression of inflammatory cytokines CRP (P≤0.05). Grifola frondosa polysaccharide intracellular polysaccharide can increase immune organs in mice, increased immune cell secretion, increased expression of related immune factors.