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以10个转TaLEA小黑杨无性系和1个对照无性系为材料,对无性系苗期苗高和地径生长过程进行测定并构建Logistic模型,探讨不同无性系苗期生长过程的变异情况。结果表明:转基因小黑杨无性系苗高和地径生长过程高度符合“S”型曲线,所有Logistic模型拟合系数超过0.9,表明拟合效果显著。方差分析结果表明不同无性系苗高和地径间差异均达到极显著水平(P<0.01)。参试无性系苗高平均速生期持续期为48天,速生期内平均生长量为1.85 cm,地径平均速生期持续时间为70天,速生期内平均生长量为0.09 mm。苗高、地径均与速生期内生长量、速生期内平均日生长量间呈极显著正相关。转基因无性系XL-1和XL-9苗期生长较快,苗高(地径)分别比对照高16.37%(32.82%)和10.92%(22.68%),表明转LEA基因对无性系生长具有一定的影响。此研究为小黑杨苗期管理提供理论依据。
Ten clones of Taole Populus clone and a control clone were used to study the seedling height and diameter growth of clones at seedling stage and to establish Logistic model to investigate the variation of seedling growth in different clones. The results showed that the height and diameter growth of the transgenic Populus tomentosa clone were highly consistent with the “S” curve and the fitting coefficients of all Logistic models were over 0.9, indicating that the fitting effect was significant. Analysis of variance showed that the differences of seedling height and diameter of different clones reached the significant level (P <0.01). The mean climacteric period of the clones was 48 days, the average growth rate was 1.85 cm in the fast-growing period, the average fast-growing period was 70 days, and the average fast-growing period was 0.09 mm. There was a significant positive correlation between the seedling height and the diameter of the soil with the growth rate during fast growth and the average daily growth rate during rapid growth. The transgenic clones XL-1 and XL-9 grew rapidly at the seedling stage, with seedling height (diameter) of 16.37% (32.82%) and 10.92% (22.68%) higher than that of the control respectively, indicating that transgene LEA has a certain clonal growth Impact. This study provides a theoretical basis for Populus simonii seedling management.