2019年10月,在日本本州中部长野县的富士山冷杉树枝及树叶上采集到4种黑斑病致病菌标本,经鉴定为已知致病菌Capnobotrys neesii(Capnobotrys的模式种).此外,通过单孢分离法培养出具有代表性的菌株JCM 39119,能产生具有瓶梗状的产孢细胞和分生孢子的念珠状菌丝、以及类似于在天然宿主上生长的分生孢子梗和小型分生孢子的菌丝.根据植物多形性真菌学名重新命名的原则,无性型类群C.neesii被划分至rnMetacapaodium,作为新组合Metacapnodium neesii.测定了JCM 39119菌株的nrLSU、nrSSU、ITS、RPB2和EFIa基因序列,并基于核糖体小亚基序列进行系统发育分析,将JCM 39119菌株定位在刺盾炱目Chaetothyriales的分类单元上,而非预期的煤炱目Capnodiales或格孢菌目Pleosporales分类单元.因此,必须进行进一步实验分析,通过对其他基因序列分析得到最终结论.本研究结果支持对以Metacapnodium为代表的假煤炱科Metacapnodiaceae的更高级别分类进行修订.“,”Four specimens of a metacapnodiaceous sooty mould occurring on living twigs and leaves of the conifer Abies veitchii were collected in October 2019 from the Sugadaira Plateau,Nagano,central Honshu,Japan.These were assigned to the known sooty mould Capnobotrys neesii,the type species of the genus Capnobotrys.In addition,a repre-sentative strain(JCM 39119)was cultured from a single conidium,producing moniliform hyphae bearing sympodial conidiogenous cells and conidia,and phialides and microconidia resembling those growing on natural hosts.Following the protocol for re-assigning the scientific name of pleomorphic fungi,the asexually-typified species C.neesii is trans-ferred to Metacapnodium as Metacapnodium neesii comb.nov.The nrLSU,nrSSU,ITS,RPB2,and EF1α gene se-quences for JCM 39119 were determined.Phylogenetic analysis based on the nrSSU DNA sequences positioned JCM 39119 at the base of the taxon Chaetothyriales,rather than Capnodiales or Pleosporales as expected.However,further assessments including other gene sequences are required to conclusively place.Our results support revision of the higher-level classification of Metacapnodiaceae typified by Metacapnodium.