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目的了解成都市不同托幼机构在园儿童手足口病常见病原携带情况,为分析手足口病发病规律、科学防控提供基础数据和科学依据。方法采用分层随机抽样,采集不同经济圈层、不同季节、城镇与农村托幼机构健康在园儿童549份咽拭子,以荧光PCR检测手足口病相关病毒:肠道病毒通用(EV)、柯萨奇病毒A16型(CA16)、肠道病毒71型(EV71)。结果核酸结果显示,549份样品中检出EV71阳性9份(阳性率1.63%),其他肠道病毒27份(阳性率4.92%),未检出CA16,总阳性率6.6%。统计学分析结果显示,城镇与农村差异不显著(χ2=0.080,P=0.777>0.05),性别差异不显著(χ2=0.097,P=0.756>0.05),但经济圈层和流行季节有差异(前者χ2=4.497,P=0.034<0.05;后者χ2=5.925,P=0.0.015<0.05)。结论成都市在园儿童手足口病病原携带率在不同经济圈层和不同季节有所差异,可能与发病有关,应有针对性的开展防控工作。
Objective To understand the prevalence of hand, foot and mouth disease in children with different nurseries in Chengdu and to provide basic data and scientific basis for the analysis of the pathogenesis of hand-foot-mouth disease and scientific prevention and control. Methods Stratified random sampling was used to collect 549 throat swabs in healthy children in different economic circles, different seasons, cities and rural nurseries. Fluorescent PCR was used to detect hand-foot-mouth disease-related viruses: Enterovirus (EV) Coxsackie virus A16 (CA16), enterovirus 71 (EV71). Results The results of nucleic acid analysis showed that 9 out of 549 samples were positive for EV71 (positive rate 1.63%), 27 other enteroviruses (positive rate 4.92%) and no positive detection rate for CA16. The total positive rate was 6.6%. The results of statistical analysis showed that there was no significant difference between urban and rural areas (χ2 = 0.080, P = 0.777> 0.05), but no significant gender difference (χ2 = 0.097, P = 0.756> 0.05) The former χ2 = 4.497, P = 0.034 <0.05; the latter χ2 = 5.925, P = 0.0.015 <0.05). Conclusion The prevalence of hand, foot and mouth disease in children in Chengdu is different in different economic circles and different seasons, which may be related to the pathogenesis. Therefore, prevention and control should be carried out in a targeted manner.