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目的 :研究新化合物特苄康唑对真菌麦角甾醇生物合成的影响。方法 :用薄层色谱扫描法考察真菌经不同浓度特苄康唑作用后 ,其麦角甾醇和羊毛甾醇的含量变化。结果 :特苄康唑能使真菌中的麦角甾醇含量下降 ,羊毛甾醇含量增加 ,两者的含量变化均有明显的剂量依赖性 ;同浓度时特苄康唑对真菌生长繁殖和麦角甾醇生物合成的抑制作用无显著差异。结论 :特苄康唑是通过抑制真菌的麦角甾醇生物合成而发挥其高效抗真菌活性的 ,为羊毛甾醇 14α 去甲基化酶抑制剂。
Objective: To study the effect of the new compound terbaconide on the ergosterol biosynthesis in fungi. Methods: The contents of ergosterol and lanosterol in fungi were determined by thin layer chromatography (TLC). Results: Terbaconazole decreased the content of ergosterol in fungi and the content of lanosterol in both groups, and the content of both were significantly dose-dependent. Terbenoconazole also had a dose-dependent effect on fungal growth and ergosterol biosynthesis No significant difference in inhibition. CONCLUSION: Terbaconide is a lanosterol 14α-demethylase inhibitor that exerts its anti-fungal activity by inhibiting the ergosterol biosynthesis of fungi.