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目的 建立可供实验研究的稳定的兔 Vx2移植性肝癌模型 ,探讨不同植瘤方式的成功率 ,并分析该肿瘤的 DSA影像特征 .方法 新西兰白兔 6 0只 ,随机分 3组 ,每组 2 0只 .将Vx2瘤细胞 (5× 10 7个 )经肝动脉或经肝包膜分别接种于 2组兔的肝左叶 ,建立对照肝癌模型 .第 3组经肝包膜植入瘤组织块 (约含 10 6～ 10 8个瘤细胞 )建立改良肝癌模型 .观察 :1不同组植瘤的成活率 ;2改良组肿瘤 7,10 ,14,17和 2 1d时的体积(B超测 ) ,并计算肿瘤生长率 ;3大体及镜下 (光镜和电镜 )瘤组织形态特征 ;4改良 Vx2移植性肝癌的 DSA影像特征 .结果 3组植瘤成活率分别为 7/ 2 0 ,10 / 2 0和 19/ 2 0 ,改良组植瘤成活率最高 (P<0 .0 5 ) ,瘤体呈指数性生长 ,组织病理及电镜表明该瘤在肝组织中浸润式生长 ,其形状与移植于兔其他部位的 Vx2鳞状细胞癌特征相似 .DSA影像示该移植性肝癌具有丰富的血供 .结论 成功建立了兔 Vx2移植性肝癌改良模型 ,瘤组织块种植方式成功率明显高于动脉途径和细胞液注射方式 ,为肝癌研究提供了大型实验模型 .
Objective To establish a stable rabbit Vx2-transplanted hepatocellular carcinoma model that can be experimentally studied, to investigate the success rate of different tumors, and to analyze the DSA imaging characteristics of the tumor. Methods New Zealand white rabbits were randomly divided into 3 groups of 2 0. Vx2 tumor cells (5×10 7) were inoculated into the left hepatic lobe of the 2 rabbits via the hepatic artery or the liver capsule to establish a control liver cancer model. The third group was transplanted into the hepatic capsular tissue block. (Contains about 106 to 108 tumor cells) Establish an improved liver cancer model. Observations: 1 survival rate of different groups of tumors; 2 volume of modified tumors on days 7, 10, 14, 17 and 21 (B-ultrasound) , And calculate the tumor growth rate; 3 General and microscopic (light and electron microscopy) tumor morphological characteristics; 4 Improved DSA image characteristics of Vx2 transplanted liver cancer. Results The survival rate of the three groups of tumors was 7 / 2 0, 10 / On the 20th and 19th/20th, the survival rate of the improved group was the highest (P<0.05). The tumor showed exponential growth. Histopathology and electron microscopy showed that the tumor had infiltrative growth in the liver tissue, its shape and transplantation. The characteristics of Vx2 squamous cell carcinoma in other parts of rabbits were similar. DSA images showed that the transplanted liver cancer had abundant blood supply. Conclusions Successfully established a rabbit Vx2 improved model transplanted liver cancer, the tumor tissue mass planting pattern was significantly higher than the success rate of arterial pathways and cellular fluid injection mode, it provides a large-scale experimental model for the study of liver cancer.