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目的 探讨膳食大豆蛋白和 或高钙摄入对高血胆固醇模型大鼠血浆胆固醇及有关血脂指标的影响。方法 用含 5 %猪油、1%胆固醇和 0 2 5 %胆碱的高脂饲料喂饲健康Wistar雌性大鼠 ,经 14d诱发高血胆固醇以后 ,测血浆总胆固醇 (TC) ,总甘油三酯 (TG) ,高密度脂蛋白胆固醇 (HDL C)和载脂蛋白B(ApoB1 0 0 )的浓度。然后 ,按体重和血浆胆固醇浓度将动物均衡分为 4组 ,以 2× 2析因设计 ,分别喂饲含有酪蛋白和大豆分离蛋白及正常钙 (0 5 9% )和高钙水平 (1 12 % )的纯合成高脂饲料。经 2 1d喂养后 ,采血 ,测定指标同前。实验结果用SPSS统计软件进行方差分析。结果 经 14d诱导高血脂后 ,实验模型大鼠的TC水平为 (4 10± 1 11)mmol L (n =2 4) ,而喂饲正常饲料的阴性对照鼠仅为 (1 92± 0 2 8)mmol L(n =8) ,升高了 2 14倍。又经 2 1d喂饲含有处理因素的饲料后 ,酪蛋白组 ,酪蛋白加钙组 ,大豆蛋白组和大豆蛋白加钙组大鼠的TC水平分别为 (10 5 6± 2 74,7 99± 2 19,5 48± 0 5 1和 6 81± 0 98)mmol L ;ApoB1 0 0 为 (0 46± 0 14,0 31± 0 12 ,0 17± 0 0 7和 0 2 1± 0 0 5 )g L。同酪蛋白组相比 ,其他 3组动物的TC和ApoB1 0 0 浓度显著降低。而各组间TG和HDL C的变化均无显著性。结论 ?
Objective To investigate the effect of dietary soy protein and high calcium on plasma cholesterol and related lipid profile in hypercholesterolemic rats. Methods Wistar female rats were fed with high fat diet containing 5% lard, 1% cholesterol and 0.52% choline. After 14 days of hypercholesterolemia, total cholesterol (TC), total triglyceride (TG), high density lipoprotein cholesterol (HDL C) and apolipoprotein B (ApoB10). The animals were then divided equally into 4 groups according to their body weight and plasma cholesterol concentrations. The animals were fed a diet containing casein and soy protein isolate, normal calcium (0 59%), and high calcium (1 12 %) Of pure synthetic high-fat feed. After 2 1d feeding, blood collection, measurement indicators with the former. The experimental results using SPSS statistical software for analysis of variance. Results After hyperlipidemia was induced for 14 days, the TC level in the experimental model rats was (4 10 ± 1 11) mmol L (n = 24), while the negative control rats fed the normal diet only (92 ± 0 8 8 ) mmol L (n = 8), a 2 14-fold increase. The TC levels of casein, casein plus calcium, soy protein and soy protein plus calcium groups were (105 6 ± 2 74,7 99 ± 2 19,5 48 ± 0 5 1 and 6 81 ± 0 98) mmol L; ApoB1 0 0 was (0 46 ± 0 14,0 31 ± 0 12,017 ± 0 0 7 and 0 2 1 ± 0 0 5 ) g L. Compared with the casein group, TC and ApoB10O concentrations in other three groups of animals were significantly reduced. The changes of TG and HDL C in each group were not significant. in conclusion ?