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目的:建立一个较理想的CCl4药物性肝损伤体外模型。方法:分别采用传统方法和改进方法配制CCl4损伤液并诱导人肝HepG2细胞损伤,倒置相差显微镜观察细胞形态学变化,生化法检测上清液中ALT水平并采用MTT法测定细胞活性。结果:改进方法的CCl4诱导损伤效果明显优于传统方法,随着CCl4损伤液浓度的提高,上清液中ALT水平明显升高,而细胞活性显著降低,70%浓度CCl4损伤液诱导损伤4 h可获得最佳损伤效果。结论:利用改进方法可在体外实验中建立较理想的CCl4药物性肝损伤模型,此模型可为进一步的体外实验研究奠定基础。
Objective: To establish a more ideal in vitro model of CCl4 drug-induced liver injury. Methods: The CCl4 injury fluid was prepared by traditional method and modified method, respectively. HepG2 cells were induced to injure. HepG2 cells were injured by morphological changes under inverted phase contrast microscope. The level of ALT in the supernatant was detected by biochemical method. The cell viability was measured by MTT assay. Results: The improved method of CCl4 induced injury was significantly better than the traditional method, with the increase of CCl4 injury fluid concentration, the supernatant ALT levels were significantly increased, while the cell activity was significantly reduced, 70% CCl4 injury liquid induced injury 4 h Get the best damage results. Conclusion: The improved CCl4 drug-induced liver injury model can be established in vitro in vitro. This model can lay the foundation for further in vitro experimental research.