论文部分内容阅读
经CSC、DEN、MNU等致癌物转化和未转化的凋亡Rat-1细胞谷胱甘肽-S-转移酶(GSTs)、谷胱甘肽-S-转移酶-π(GST-π)活性均较其相应的未凋亡细胞显著增高(P<0.05~0.01)。经Northern杂交证实,上述凋亡细胞GST-πmRNA表达水平也增高,这可能是导致GSTs和GST-π活性增高的主要原因。由于GSTs可催化谷胱甘肽(GSH)与众多亲电子物质结合而有重要解毒功能,故推测培养细胞因营养匮乏引起凋亡时,其自身代谢产生的有毒物质无法及时被清除而积聚,可能启动了细胞自身的防御体系,行使自我保卫功能,从而刺激GSTs活性增高。
After CSC, DEN, MNU and other carcinogen transformation and non-transformation of apoptotic Rat-1 cells glutathione-S-transferase (GSTs), glutathione-S-transferase-π Compared with their corresponding non-apoptotic cells were significantly higher (P <0.05 ~ 0.01). Northern blot showed that the expression of GST-πmRNA also increased, which may be the main reason leading to the increase of GSTs and GST-πactivity. Since GSTs can catalyze the combination of glutathione (GSH) with many electrophilic substances and have important detoxification functions, it is speculated that when cultured cells are apoptosis due to nutrient deficiency, the accumulated toxic substances generated by their own metabolism may not accumulate in time and may be accumulated Start the cell’s own defense system, exercise self-defense function, thereby stimulating GSTs increased activity.