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目的 观察不同大小的 + Gz暴露对大鼠脑皮层 HSP70 m RNA表达水平的影响 ,探讨+ Gz暴露致脑损伤的机制。 方法 80只 SD大鼠随机分为对照组、+ 2 Gz暴露组、+ 6Gz暴露组和+ 10 Gz暴露组。各组在 G暴露后的 5 h、10 h、1d、2 d和 4d分批处死 ,采用原位分子杂交方法观察大鼠脑皮层 HSP70 m RNA表达情况 ,并对实验结果进行统计分析。 结果 3个 + Gz暴露组 ,HSP70m RNA表达水平表现出相同的变化趋势 :即在 + Gz暴露后 5 h,HSP70 m RNA表达水平开始增加 ,10 h达峰值 ,4d时基本恢复正常 ,而对照组没有这一变化趋势。不同 G值之间的比较表明 ,+ 6Gz组HSP70 m RNA总体表达水平最高 ,且分布较广泛 ,+ 10 Gz 组最低 ,且分布较局限 ,主要位于靠近颅底部的皮层中。 结论 + Gz暴露对大鼠脑皮层 HSP70 m RNA表达水平有着显著的影响
Objective To observe the effect of different + Gz exposure on HSP70mRNA expression in rat cerebral cortex and to explore the mechanism of + Gz exposure induced brain injury. Methods Eighty SD rats were randomly divided into control group, +2 Gz exposure group, +6 Gz exposure group and +10 Gz exposure group. The rats in each group were sacrificed at 5 h, 10 h, 1 d, 2 d and 4 d after G exposure. The expression of HSP70 mRNA in the cerebral cortex was observed by in situ hybridization. The experimental results were statistically analyzed. Results The expression of HSP70mRNA showed the same trend in 3 + Gz exposure groups: HSP70mRNA expression began to increase at 5h after + Gz exposure, peaked at 10h and returned to normal at 4d, while in control group There is no such trend. The comparison of different G values showed that the expression level of HSP70 mRNA in + 6Gz group was the highest, with a wide distribution, the lowest in +10 Gz group, and the distribution was limited, mainly located in the cortex near the skull base. Conclusion + Gz exposure has a significant effect on HSP70 mRNA expression in rat cortex