[目的]测定4.8%鱼藤酮.辣椒碱悬浮剂对蜜蜂、鹌鹑、斑马鱼和家蚕4种生物的半数致死量,评价其安全性。[结果]4.8%鱼藤酮.辣椒碱悬浮剂对蜜蜂、雌(雄)鹌鹑、斑马鱼和家蚕LD50值分别为271.2、40.9(35.6)、0.0116、3.58 mg/L。[结论]4.8%鱼藤酮.辣椒碱悬浮剂对蜜蜂低毒,在实际使用过程中对鸟类急性中毒风险较小。对家蚕高毒,对鱼类剧毒,使用时远离桑园,在桑园附近农田使用时注意施药方式和风向,避免药液飘落到桑叶上。要严格避免药液流入水域对鱼类造成危害。 [Objective] To determine the half-lethal dose of 4.8% rotenone-capsaicin suspension to bee, quail, zebrafish and silkworm, and evaluate their safety. [Result] The LD50 values ​​of 4.8% rotenone and capsaicin suspension for bees, female (male) quails, zebrafish and silkworm were 271.2, 40.9 (35.6), 0.0116 and 3.58 mg / L, respectively. [Conclusion] 4.8% rotenone and capsaicin suspending agent have low toxicity to honey bees, and the risk of acute poisoning of birds in practical use is relatively low. Highly toxic to silkworms, highly toxic to fish, away from the mulberry use, use of farmland near the Mulberry attention to the way and direction of application of pesticides to prevent falling to the mulberry leaves. To strictly avoid the liquid into the waters of fish hazards.