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目的:观察枳术汤对脾虚滞结便秘小鼠5-羟色胺受体4(5-HT4-R)和生长抑素(SS)基因表达的影响。方法:复制脾虚滞结便秘小鼠模型,造模组分别给予枳术汤、麻仁软胶囊、生理盐水治疗,用RT-PCR法检测并比较各组小鼠胃肠道5-羟色胺受体4和生长抑素mRNA的含量。结果:模型小鼠5-HT4-R含量较空白组降低,尤以胃窦降低明显,而SS较空白组升高,以胃窦升高明显;治疗后枳术汤组5-HT4-R含量较其他组升高,而SS较其他组降低。结论:枳术汤可提高脾虚滞结便秘小鼠胃窦5-HT4-R基因的表达,降低胃窦SS的表达。
OBJECTIVE: To observe the effect of Qizhu Decoction on the expression of 5-HT4-R and somatostatin (SS) genes in spleen stagnation and constipation in mice. Methods: The mouse models of spleen deficiency and stagnation and constipation were duplicated. The model group was treated with Zhizhu Decoction, Mare soft capsule and normal saline respectively. The gastrointestinal serotonin receptor 4 in each group was detected and compared by RT-PCR. And somatostatin mRNA content. Results: The 5-HT4-R content in the model mice was lower than that in the blank group, especially gastric antrum was significantly lower, SS was higher than the blank group, and the gastric antrum was significantly elevated; 5-HT4-R content in Qizhu Decoction group after treatment. Compared with other groups, SS was lower than other groups. Conclusion: Qizhu Decoction can increase the expression of 5-HT4-R gene in stomach antrum and decrease the expression of SS in gastric antrum.