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背景:活体示踪移植干细胞,影像学评估移植细胞动态变化的趋势是目前的热点研究方向。目的:探讨MRI活体示踪超顺磁性氧化铁标记的骨髓间充质干细胞移植修复心肌的可行性及有效性。方法:经导管应用球囊封堵前降支动脉建立家猪心肌梗死模型,造模成功的动物随机分为2组:实验组在造模2周后于梗死区中央及梗死边缘区共5个点注射超顺磁性氧化铁-多聚赖氨酸混合物标记的骨髓间充质干细胞悬液1mL,对照组以同样方法注入等量的生理盐水。结果与结论:细胞移植后1h,2周,实验组中4只动物(100%)移植区在磁共振上呈明显的低信号改变,移植后4周示3只动物(75%)细胞移植区有低信号改变。移植后4周,心肌组织荧光免疫组化检测心脏结蛋白、心脏连接蛋白和心肌早期转录蛋白均有表达。结果可见应用MRI技术活体示踪动态观察移植的骨髓间充质干细胞是可行有效的。
BACKGROUND: The trend of dynamic changes of transplanted cells assessed by in vivo tracing of stem cells and imaging is the current hot research direction. OBJECTIVE: To investigate the feasibility and effectiveness of MRI biopsy with superparamagnetic iron oxide-labeled bone marrow mesenchymal stem cells for myocardial repair. Methods: The porcine model of myocardial infarction was established by occluding the anterior descending artery via balloon catheterization. The successful animals were randomly divided into 2 groups: experimental group in the central infarction zone and infarct border zone 5 weeks after modeling Point injection of superparamagnetic iron oxide - polylysine mixture marked bone marrow mesenchymal stem cell suspension 1mL, the control group in the same way by injecting an equal amount of saline. RESULTS AND CONCLUSION: After 1 and 2 weeks of cell transplantation, 4 animals (100%) in the experimental group showed obvious low signal changes in MRI. Three animals (75%) were implanted in the transplantation area There is a low signal change. Four weeks after transplantation, the expression of cardiac knot protein, cardiac connexin and early myocardial transcription protein were detected by fluorescence immunohistochemistry. The results show that the use of MRI technology in vivo dynamic tracking of transplanted bone marrow mesenchymal stem cells is feasible and effective.