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目的：研究重组人白细胞介素６（ｒｈＩＬ－６）对原代培养大鼠肝细胞分泌纤维蛋白原（Ｆｉｂｒｉｎｏｇｅｎ，Ｆｇｎ）的影响及蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｒｏ３１－８２２０对枯否细胞分泌ＩＬ－６的作用。方法：以酶联免疫吸附测定法（ＥＬＩＳＡ）测定Ｆｇｎ；测定Ｉ－６采用［３Ｈ］－ＴｄＲ掺入增殖法。结果：大鼠肝细胞（３×１Ｏ６／ｍｌ）与ｒｈＩＬ－６（０～１００ｋＵ／Ｌ）孵育２４ｈ，刺激肝细胞分泌Ｆｇｎ增加；Ｒｏ３１－８２２０（０．１～１０μｍｏｌ／Ｌ）显著抑制枯否细胞释放ＩＬ－６。结论：提示ＩＬ－６是调节纤维蛋白原合成的重要细胞因子，推测ＰＫＣ抑制剂可通过抑制枯否细胞分泌ＩＬ－６来降低Ｆｇｎ的分泌。
AIM: To investigate the effect of recombinant human interleukin-6 (IL-6) on the fibrinogen (Fgn) secretion of primary cultured rat hepatocytes and the effect of protein kinase C (PKC) inhibitor Ro31-8220 on the secretion of Kupffer cells The role of IL-6. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to determine Fgn. I-6 was assayed by [3H] -TdR incorporation assay. Results: After incubated with 3 × 10 6 / ml rhIL-6 (0-100 kU / L) for 24 hours, the hepatocytes secreted Fgn increased. Ro31-8220 (0.1-10 μmol / L) Cells release IL-6. CONCLUSION: It is suggested that IL-6 is an important cytokine that regulates fibrinogen synthesis. It is speculated that PKC inhibitor can decrease Fgn secretion by inhibiting the secretion of IL-6 from Kupffer cells.