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采用丙烯酰胺垂直平板电泳法，对天冬氨酸转氨酶（ＧＯＴ）、丙氨酸氨酸酶（ＡＡＰ）、亮氨酸氨酸酶（ＬＡＰ）、６－磷酸葡萄糖酶（６０ＧＤ）、磷酸葡萄糖变位酶（ＰＧＭ）、莽草酸脱氢酶（ＳＨＤＨ）等 ６种同工酶系统的 １２个基因位点进行了研究和分析，共发现 ３６个复等位基因。结果还表明，马尾松天然群体具有较为丰富的遗传变异性，其多态位点百分率（ＰＬ）为５６％，平均杂合率（Ｈｅ）为０．２８５，等位基因平均数（Ｎａ）为２．２９；有效等位基因平均数（Ｎｅ）为１．４２；马尾松天然群体间的变异量只占总变异量的８．８％左右，而约９１％以上的变异只存在于单个的小群体内的单株间；湖北省马尾松天然群体内存在较为严重的近亲交配现象，近交系数（Ｆｉｓ）平均为 ０．２０２。
The activity of aspartate aminotransferase (GOT), alanine aminotransferase (AAP), leucine aminase (LAP), 6-phosphogluconate (60GD) 12 gene loci of 6 isoenzyme systems, including PGM, SHDH, were studied and analyzed. A total of 36 complex alleles were found. The results also showed that the natural population of Pinus massoniana was rich in genetic variability. The percentage of polymorphic loci (PL) was 56%, the average heterozygosity (He) was 0.285, and the average number of alleles 2.29; the average number of effective alleles (Ne) was 1.42; the variation of natural population of Pinus massoniana only accounted for about 8.8% of the total variance, while about 91% of the variation existed only in single There were more serious inbreeding in the natural population of Masson pine in Hubei Province, with an average inbreeding coefficient (Fis) of 0.202.