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本文采用反相高效液相色谱法测定天麻胶囊中天麻素的含量。色谱柱:岛津Shim—pack CLC—ODS柱,150mm×4.6mm。流动相:0.005mol/L磷酸二氢钠和磷酸氢二钾水溶液,流速1.0ml/min,检测波长220mm。结果表明天麻素在0.125mg/ml～2.5mg/ml范围内峰面积与浓度成良好线性关系,回收率为97.63％～102.73％,RSD为0.95％～2.33％(n=5)。本法简便、准确,可用于该制剂的质量控制。
This article uses reversed-phase high performance liquid chromatography to determine the content of gastrodin in Tianma capsules. Column: Shimadzu Shim-pack CLC-ODS column, 150mm x 4.6mm. Mobile phase: 0.005 mol/L sodium dihydrogen phosphate and dipotassium hydrogen phosphate aqueous solution, flow rate 1.0 ml/min, detection wavelength 220 mm. The results showed that the peak area and concentration of gastrodin in the range of 0.125mg/ml to 2.5mg/ml showed a good linear relationship, the recovery was 97.63% to 102.73%, RSD was 0.95% to 2.33% (n=5). This method is simple and accurate and can be used for quality control of the preparation.