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一、HBV和HBV相关标记物在感染了肝炎病毒的人和黑猩猩的血液中存在3种颗粒:HBV的颗粒是被称作Dane颗粒的直径42nm的球型颗粒,双层构造;内部核心的core颗粒,直径27nm,显示HBc抗原活性。将此颗粒用2ME(2巯基乙醇)和SDS(十二烷基硫酸纳)处理后,可产生新的HBe抗原活性,包含核心的衣壳所显示的抗原活性称HBs活性。当HBV在肝细胞中增殖时,这个衣壳部分即产生过量,以管型颗粒和小球型颗粒状和Dane颗粒的几十倍到几千倍的量释放入循环血液中。构成HBs抗原的多肽能分解为数条,其中p22’gp27两条是主要的,它们同时在HBVDNA的S基因架编码,由226个氨基酸残基构成。p22和gp27在分子量上的差别是由于N末端第146位天门冬酰氨残基上结合的糖链不同,gp31,gp35是gp27、p22N末端第55个氨基酸所结合的,已明确,
First, HBV and HBV-related markers There are three kinds of particles in the blood of people infected with hepatitis virus and chimpanzees: HBV particles are spherical particles with a diameter of 42 nm called Dane particles, a double-layer structure; the core of the inner core Particles, 27 nm in diameter, show HBc antigen activity. After the pellet was treated with 2 ME (2 mercaptoethanol) and SDS (sodium lauryl sulfate), a new HBe antigenic activity was produced, and the antigenic activity exhibited by the core-containing capsid was termed HBs activity. When HBV is multiplied in hepatocytes, this part of the capsid produces an excess and is released into the circulating blood in an amount of several tens to several thousand times the number of the tube-shaped particles and the bead-shaped particles and the Dane particles. Polypeptides that make up the HBs antigen can be broken down into several bands, of which p22’gp27 is the main one. They are both encoded by the S gene of HBVDNA and consist of 226 amino acid residues. The differences in molecular weight between p22 and gp27 are due to the different sugar chains bound to the asparagine residue at the N-terminal position 146, and gp31 and gp35 are the binding sites of gp27 and the 55th amino acid at the p22 N terminus.