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目的探讨维替泊芬对MDA-MB-231乳腺癌细胞增殖、侵袭和迁移的影响及其机制。方法选取对数生长期的MDA-MB-231细胞,随机分为对照组和维替泊芬处理组。先采用CCK-8法确定维替泊芬对MDA-MB-231细胞增殖抑制的最低抑制浓度,而后采用4μmol/m L维替泊芬处理MDA-MB-231细胞,采用TranswellTM侵袭实验检测细胞的侵袭能力,采用划痕实验检测细胞迁移能力;采用Western blot法检测(0、4、8、12、16)μmol/m L维替泊芬对MDA-MB-231细胞中增殖相关蛋白c-MYC、细胞周期蛋白D1(cyclin D1)、Yes相关蛋白(YAP)及其靶基因富半胱氨酸蛋白61(CYR61)和结缔组织生长因子(CTGF)的蛋白表达水平。结果维替泊芬明显抑制MDA-MB-231细胞的增殖,且呈剂量依赖性,最低抑制浓度为4μmol/m L。4μmol/m L维替泊芬显著抑制MDA-MB-231细胞的侵袭及迁移。维替泊芬下调MDA-MB-231细胞中增殖相关蛋白c-MYC、细胞周期蛋白cyclin D1及YAP、CYR61和CTGF的蛋白表达水平。结论维替泊芬通过下调YAP及其靶基因CYR61、CTGF表达显著抑制MDA-MB-231细胞的增殖、侵袭及迁移。
Objective To investigate the effect of verteporfin on the proliferation, invasion and migration of MDA-MB-231 breast cancer cells and its mechanism. Methods MDA-MB-231 cells in logarithmic growth phase were selected and randomly divided into control group and verteporfin-treated group. The minimal inhibitory concentration of verteporfin on MDA-MB-231 cells was determined by CCK-8 method. MDA-MB-231 cells were treated with 4 μmol / m L of verteporfin and the cell viability Invasion ability and cell migration ability by scratch assay. Western blot was used to detect the effect of (0,4,8,12,16) μmol / m L verteporfin on proliferation-associated protein c-MYC Cyclin D1, Yes-related protein (YAP) and its target genes cysteine protein 61 (CYR61) and connective tissue growth factor (CTGF). Results Verteporfin significantly inhibited the proliferation of MDA-MB-231 cells in a dose-dependent manner with a minimum inhibitory concentration of 4μmol / Verteporfin at 4μmol / m L significantly inhibited the invasion and migration of MDA-MB-231 cells. Verteporfin downregulated the protein levels of c-MYC, cyclin D1, YAP, CYR61 and CTGF in MDA-MB-231 cells. Conclusion Verteporfin can significantly inhibit the proliferation, invasion and migration of MDA-MB-231 cells by down-regulating YAP and its target genes CYR61 and CTGF.