论文部分内容阅读
目的:通过检测小细胞肺癌(small cell lung carcinoma,SCLC)和低分化鳞状细胞癌(poorly differentiated squamous-cell carcinoma,PDSCC)中FLICE抑制蛋白(FLICE inhibitory protein,FLIP)、促凋亡蛋白Smac(second mitochondrial activator of caspase,Smac)、抗凋亡因子survivin的表达,探讨小细胞肺癌及低分化鳞癌中FLIP、Smac和survivin蛋白的表达规律及其与临床病理指标及预后的关系,为临床诊断、治疗及预后判断提供新的理论依据。方法:应用免疫组化S-P法检测肺小细胞肺癌及低分化鳞状细胞癌中FLIP、Smac和survivin阳性表达;应用蛋白印记(Western blot)和逆转录-聚合酶链反应(RT-PCR)检测正常肺组织及两种癌组织中FLIP、Smac、survivin蛋白和mRNA表达水平。结果:73例肺癌患者1年生存率为64.5%。其中小细胞肺癌60.5%,低分化鳞状细胞癌68.6%。FLIP、Smac及survivin在小细胞肺癌及低分化鳞癌中的表达均与年龄、性别、是否吸烟及淋巴结转移无关(P>0.05),但Smac的表达与临床分期及生存率有关(P<0.05);Smac在正常组织表达显著高于癌组织(P<0.05);FLIP及survivin在正常组织中的表达显著低于癌组织(P<0.05)。结论:癌组织中Smac低表达提示低生存率,Smac可作为独立预后指标;FLIP、Smac和survivin联合检测有助于预后判断。
OBJECTIVE: To detect the expression of FLICE, FLIP and Smac in small cell lung carcinoma (SCLC) and poorly differentiated squamous-cell carcinoma (PDSCC) To investigate the expression of FLIP, Smac and survivin in small cell lung cancer (NSCLC) and poorly differentiated squamous cell carcinoma (SCC) and their relationship with clinicopathological parameters and prognosis, , Treatment and prognosis to provide a new theoretical basis. Methods: The expressions of FLIP, Smac and survivin in small cell lung cancer and poorly differentiated squamous cell carcinoma were detected by immunohistochemical SP method. The expression of FLIP, Smac, survivin protein and mRNA in normal lung tissue and two kinds of cancerous tissues. Results: The one-year survival rate of 73 lung cancer patients was 64.5%. Including small cell lung cancer 60.5%, poorly differentiated squamous cell carcinoma 68.6%. The expression of FLIP, Smac and survivin in small cell lung cancer and poorly differentiated squamous cell carcinoma were not related to age, sex, smoking or lymph node metastasis (P> 0.05), but the expression of Smac was correlated with clinical stage and survival rate (P <0.05 ). The expression of Smac in normal tissues was significantly higher than that in cancer tissues (P <0.05). The expression of FLIP and survivin in normal tissues was significantly lower than that in cancerous tissues (P <0.05). Conclusion: The low expression of Smac in cancerous tissues suggests a low survival rate, and Smac may be used as an independent prognostic factor. Combined detection of FLIP, Smac and survivin may be helpful for prognosis.